Proteomics Analysis Supporting Lab

This laboratory is a platform for in gel proteome separation by two-dimensional gel electrophoresis (2-DE) and respective digital analysis, under the coordination of Pedro Santos and Pedro Castro. With 2-DE, proteins are fractionated according to two characteristics:

i) charge (isoelectric point, pI) and ii) molecular mass, resulting in the separation of complex protein extracts in hundreds to a few thousands of protein spots within one gel. The 2-D separation of proteins allows not only the study of protein levels (spot intensity), but also the detection of isoforms, protein modifications (spot position), and study protein interactions and complexes (native electrophoresis).

This platform is mainly suitable for high-throughput screening of proteome dynamics throughout different experimental conditions and time-points, in both prokaryotic and eukaryotic organisms.