The BactoCast project focuses on developing strategies that facilitate the establishment of young chestnut trees in the field. By identifying the microbial diversity of the roots of chestnut trees from Trás-os-Montes, the project aims to explore and enhance this diversity, promoting mycorrhization assisted by bacteria. Due to the benefits of mycorrhization, this innovative idea will allow for the expansion of the chestnut tree's cultivation area, its resistance to stress, and its chestnut production capacity.