People Detail

2005: PhD in Biochemistry. Project title: A novel family 8 psychrophilic xylanase: fundamentals and applications. PhD project completed at the Laboratory of Biochemistry, University of Liege, Belgium under the supervision of Prof. Charles Gerday. 2005-2007: Post-doctoral study at the University of Liege developing cold-adapted enzymes for use in the food and feed industries. Project title: Fundamental and applied aspects of psychrophilic xylanases and cross-linking enzymes. 2007-2009: EMBO long-term fellowship at the laboratory of Cell Physiology and NMR headed by Prof. Helena Santos at the ITQB, Univ. Nova de Lisboa, Lisbon, Portugal. Project Title: NMR solution structure and dynamics of DsbA enzymes. 2009 - Present Day: FCT researcher at the CBMA. Involved in research, training and teaching activities. Publications (15/01/2020): 57; Published International Patents: 2; Citations: 5261. Internationally Commercialised Biotechnological Products: 1 (principal investigator and inventor of a xylanase enzyme for use in the food industry, currently being marketed worldwide by Puratos N.V.). https://scholar.google.pt/citations?user=JIAMor4AAAAJ&hl=en

Expertise in various areas of protein science; from protein bioprospection, screening, production (recombinant; batch and fed-batch), purification (chromatographic and non-chromatographic), characterisation (structure, kinetics, stability, specificity) and engineering (site-directed and random mutagenesis), to the development of enzymes for application in industry. The principal objectives of our research are: 1) to identify the molecular determinants of enzyme adaptation. This involves comparative studies of the structure-function-activity-stability-dynamics of various enzymes adapted to various extreme environments as well as protein engineering to investigate the structural determinants of adaptation. 2) to develop the use of enzyme technology in industry. Investigate the use of various enzymes, principally cold-adapted enzymes, for use in various applications, especially in the food-feed industry and in the transformation of biomass to high value products. 3) to develop the enzyme/protein bioprospection toolbox. Development of advanced techniques in protein engineering and metagenomics for enzyme screening as well as optimisation of protein production approaches (batch and fed-batch).

- Enzyme Technology for Biomass Valorisation. Developing the use of enzymes for production of value-added products (functional food/feed ingredients) from biomass.
- Enhancing the Protein Engineering Toolbox: Multi-Site-Directed Mutagenesis. Developing novel tools for a more simplified and rapid protein engineering.
- Protein Engineering: Investigating the Role of Exposed Surface Residues in Protein Solubility.

EcoAgriFood: Innovative Green Products and Processes to Promote Agri-Food Bioeconomy. NORTE-01-0145-FEDER-000009. Norte2020, 2016-2019.
FunBioPlas: Novel synthetic biocomposites for biomedical devices. ERA-IB-2-6/004/2014. ERA-NET, 011016-300919, 2016-2019.

EngXyl: Unravelling the determinants of xylanase function and stability and enhancing the biotechnological value of a highly active Antarctic xylanase. EXPL/BBB-BIO/1772/2013, Projetos Exploratórios de IC&DT 2013, Fundação para a Ciência e Tecnologia (FCT), Portugal, 2014 – 2015.

NMR Net - National Facility for Nuclear Magnetic Resonance: from Molecular Structure and Dynamics to Protein Function, Cell Physiology, and Metabolomics. RECI/BBB-BQB/0230/2012, Projetos de ICDT 2012, Fundação para a Ciência e Tecnologia (FCT), Portugal, 2013 – 2016.

ECOplast: Research in new biomass-based composites from renewable resources with improved properties for vehicle parts moulding. FP7-NMP-2009-SME-3, collaborative project number 246176, European Commission funded FP7 project, 2010-2014.

Psychrophiles and psychrophilic enzymes for the study of compatible solutes and their mechanism of action: the role of protein dynamics. ALTF 950-2006, European Molecular Biology Organisation (EMBO), Heidelberg, Germany, 2007-2009.

Cold-adapted cross-linking enzymes in the food and feed industries (PINDENZ). First Europe Objectif 3, Exercice 2003, EPH3310300R0202, Direction Generale des Technologies de la Recherche et de l’Energie (DGTRE), Belgium, 2005-2007.

Development of a xylanase for use in the food industry (PINDXYL). First Europe Objectif 3, Exercice 2002(2), R0202/215266, Direction Generale des Technologies de la Recherche et de l’Energie (DGTRE), Belgium, 2003-2005

Supervision: PhD Projects Ongoing
- Mário Jorge Faria Barroca, PhD in Applied and Environmental Microbiology, University of Minho, Braga, Portugal. Project title: Enhancement of the biotechnological value of a commercial cold-adapted xylanase. Ongoing.
- Augusto Alexandre Costa Barbosa, PhD in Molecular and Environmental Biology, University of Minho, Braga, Portugal. Project title: New generation antigen delivery system for Candida albicans recombinant proteins as a vaccination strategy against systemic fungal infections. Ongoing.

Supervision: MSc. Projects Ongoing
- Mauro André Mendonça Da Cruz, MSc. in Molecular Genetics, University of Minho, 2018-2019. Protein engineering: multi-site-directed mutagenesis for enhancing protein performance.
Ricardo Daniel Gavina Gonçalves, MSc. in Molecular Genetics, University of Minho, 2018-2019. Enzyme Technology for Biomass Valorisation.

Supervision: Post-Doctoral Projects Completed
- Anne Hoyoux, University of Liege, Belgium. Development of a xylanase for use in the food industry (PINDXYL). 2003-2006.

Supervision: Research Grantees Completed
- Mário Jorge Faria Barroca, University of Minho, Braga, Portugal. 2014-2015. Project: Unravelling the determinants of xylanase function and stability and enhancing the biotechnological value of a highly active Antarctic xylanase. Research Grantee, FCT Exploratory Project EXPL/BBB-BIO/1772/2013.

Supervision: MSc. Projects Completed
- Ricardo José da Silva Cruz Pereira Faria, MSc. in Applied Biochemistry, University of Minho. 17/12/2018. Novel enzymes for the agro-food industries.
- Sofia Granja Martins, MSc. in Molecular Genetics, University of Minho, 24/01/2018. Metagenomics for Enzyme Bioprospection.
- Ana Margarida Ribeiro Pacheco, MSc. in Applied Biochemistry, University of Minho, Braga, Portugal. 19/12/2017. Deciphering the role of exposed surface residues in protein stability.
- Paulo Ruben Fernandes Veloso, MSc. in Molecular Genetics, University of Minho, 20/04/2017. Enzyme engineering for improved xylanase applicability and characterization of a xylan metabolizing Saccharomyces cerevisiae.
- Augusto Alexandre Costa Barbosa, MSc. in Molecular Genetics, University of Minho, Braga, Portugal, 01/03/2017. Development of a novel recombinant subunit antigen as a vaccination target against candidiasis: Cht3 from Candida albicans.
- Gustavo de Almeida Santos, MSc. in Molecular Genetics, University of Minho, Braga, Portugal. 2015. Engineering a commercial cold-adapted xylanase for improved low pH stability.
- Diogo Ribeiro Silva, MSc. in Molecular Genetics, University of Minho, Braga, Portugal. 2015. Understanding adaptation to pH in an industrially relevant glycoside hydrolase family 8 Xylanase
- Paulo Rodrigues, MSc. in Applied Biochemistry, University of Minho, Braga, Portugal. 2014. Genetic approaches for optimising the fed-batch production of a silk elastin-like polymer in E. coli.
- Mário Barroca, MSc. in Biophysics and Bionanosystems., University of Minho, Braga, Portugal. 2013. Optimisation of the fed-batch production of a silk-elastin-like protein: overcoming acetate accumulation and plasmid instability.
- Catherine Oliveira Ferreira, MSc. in Molecular Genetics, University of Minho, Braga, Portugal. 2012. Thiol-disulphide oxidoreductases: production, purification and structural analysis of a cold adapted DsbA.
- Frederic Roulling, MSc., University of Liege, Liege, Belgium. 2007. Study of a blue oxidase from the white continent.
- Virginia Brancato, MSc., University of Liege, Liege, Belgium. 2006. pH stability, activity and random mutagenesis of a psychrophilic xylanase.
- Florian Gillotin, MSc., University of Liege, Liege, Belgium, 2005. Study of the activity and stability of a psychrophilic xylanase as a function of pH.

Supervision: BSc. Projects Completed
- Ana Sofia de Oliveira e Silva, BSc. in Applied Biology. University of Minho, Braga, Portugal. 2013. Production, purification and development of an activity assay for a cold adapted DsbA.
- João M. Cunha Ribeiro, BSc. in Applied Biology, University of Minho, Braga, Portugal. 2013. Optimisation of the production of a silk-elastin-like polymer for use in the automobile industry.
- Sophie Leclercq, BSc., University of Liege, Liege, Belgium.2003. Study of a family 8 xylanase: stability and study of the catalytic residues.
- David Gustin, BSc. University of Liege, Liege, Belgium. 2003. Study of a psychrophilic xylanase: thermostabilisation and study of the active site.

Collins T. and Margesin R. (2019). Psychrophilic lifestyles: mechanisms of adaptation and biotechnological tools. Applied Microbiology and Biotechnology. 103(7):2857-2871. ISSN 0175-7598, DOI: 10.1007/s00253-019-09659-5. For access via Springer see: https://rdcu.be/blDtX or https://link.springer.com/article/10.1007/s00253-019-09659-5

Margesin R. and Collins T. (2019). Microbial ecology of the cryosphere (glacial and permafrost habitats): current knowledge. Applied Microbiology and Biotechnology. 103(6):2537–2549. ISSN 0175-7598, DOI: 10.1007/s00253-019-09631-3

Monteiro B., Ferraz P., Barroca M., da Cruz S.H., Collins T., Lucas C. (2018). Conditions promoting effective very high gravity sugarcane juice fermentation. Biotechnology for biofuels, 11(1):251. DOI:10.1186/s13068-018-1239-0

Silva P.C., Domingues L., Collins T., Oliveira R. and Johansson B. (2018). Quantitative assessment of DNA damage in the industrial ethanol production strain Saccharomyces cerevisiae PE-2. FEMS yeast research, 18(8), 1 December 2018, foy101. DOI:10.1093/femsyr/foy101.

Collins T. and Gerday C. (2017). Enzyme catalysis in psychrophiles. In Margesin R., (Eds), Psychrophiles: From Biodiversity to Biotechnology, Springer International Publishing, Heidelberg, Germany. Ch. 10, pp. 209-235, Edition 2, ISBN: 978-3-319-57056-3, DOI: 10.1007/978-3-319-57057-0_10.

Barroca M., Santos G., Gerday C. and Collins T. (2017). Biotechnological aspects of cold-active enzymes. In Margesin R., (Eds), Psychrophiles: From Biodiversity to Biotechnology, Springer International Publishing, Heidelberg, Germany. Ch. 19, pp.461-475, Edition 2, ISBN 978-3-319-57056-3, DOI: 10.1007/978-3-319-57057-0_19.

Silva D., Santos G., Barroca M. and Collins T. (2017). Inverse PCR for Point Mutation Introduction. In Domingues L. (Ed.) PCR: Methods and Protocols, Series Methods in Molecular Biology. Springer Science + Business Media LLC, New York. Series Vol. 1620. Ch. 5, pp. 87-100, ISBN 978-1-4939-7059-9, DOI: 10.1007/978-1-4939-7060-5_5, Series ISSN 1064-3745.

Barroca M., Santos G., Johansson B., Gillotin F., Feller G. and Collins T. (2017). Deciphering the factors defining the pH-dependence of a commercial glycoside hydrolase family 8 enzyme. Enzyme and Microbial Technology, Jan 2017. 96: 163–169. Epub 17/10/16. ISSN 0141-0229. DOI: 10.1016/j.enzmictec.2016.10.011.

Pereira A.M., Machado R., Costa A., Ribeiro A., Bernardo T.C., Collins T., Gomes A.C., Leonor I., Kaplan D.L., Reis R.L. and Casal M. (2017). Silk-based biomaterials functionalized with fibronectin type II promotes cell adhesion. Acta Biomaterialia. Jan 1; 47:50-59. Epub 2016 Oct3. DOI: 10.1016/j.actbio.2016.10.002.

Barroca M., Rodrigues P., Sobral R., Costa M.M.R., Chaves S.R., Machado R., Casal M. and Collins T. (2016). Antibiotic free selection for the high level biosynthesis of a silk-elastin-like protein. Scientific Reports, 6, 39329; DOI: 10.1038/srep39329.

Roulling F., Godin A., Cipolla A., Collins T., Miyazaki K. and Feller G. (2016). Activity–stability relationships revisited in blue oxidases catalyzing electron transfer at extreme temperatures. Extremophiles, 20(5):621-9. DOI: 10.1007/s00792-016-0851-9.

Machado R., Costa A., Sencadas V., Pereira A.M., Collins T., Rodríguez-Cabello J.C., Lanceros-Méndez S. and Casal M. (2015). Exploring the Properties of Genetically Engineered Silk-Elastin-Like Protein Films. Macromolecular Bioscience, 15, 1698–1709. DOI: 10.1002/mabi.201500132.

Costa A., Machado M., Ribeiro A., Collins T., Viruthachalam T., Neves-Petersen M.T., Rodríguez-Cabello J.C., Gomes A.C. and Casal M. (2015). Development of Elastin-Like Recombinamer Films with Antimicrobial Activity. Biomacromolecules, 16, 625−635. Epub 2015/01/12. DOI: 10.1021/bm5016706

Carneiro C., Correia A., Collins T., Vilanova M., Pais C., Gomes A.C., Real Oliveira M.E.C.D. and Sampaio P. (2015). DODAB:monoolein liposomes containing Candida albicans cell wall surface proteins: a novel adjuvant and delivery system. European Journal of Pharmaceutics and Biopharmaceutics, 89(2015),190–200. DOI: 10.1016/j.ejpb.2014.11.028.

Collins T., Barroca M., Branca F., Padrão J., Machado R. and Casal M. (2014). High Level Biosynthesis of a Silk-Elastin-like Protein in E. coli. Biomacromolecules, 15(7):2701-8. Epub 2014/06/03. DOI: org/10.1021/bm5005564.

Collins T., Barroca M., Branca F., Azevedo-Silva J., da Costa A., Machado R. and Casal M. (2013). Optimising a cell factory system for the bioproduction of silk-elastin-like polymers. Proceedings of the 1st International Conference on Natural Fibres (ICNF2013): Sustainable Materials for Advanced Applications, pp. 127. Fangueiro R. (Ed.), Guimarães, Portugal. ISBN: 978-989-20-3872-8.

Machado R., da Costa A., Sencadas V., Azevedo-Silva J., Collins T., Arias F.J., Lanceros-Mendez S., Rodríguez-Cabello J.C. and Casal M. (2013). Synthesis and processing of recombinant silk and elastin copolymers. Proceedings of the 1st International Conference on Natural Fibres (ICNF2013): Sustainable Materials for Advanced Applications, pp. 153. Fangueiro R. (Ed.), Guimarães, Portugal. ISBN: 978-989-20-3872-8.

Campos A.R, Oliveira B., Machado R., Collins T., Azevedo-Silva J., da Costa A., Immonen K., Wikström L. and Casal M. (2013). Novel nanocellulose reinforced recombinant protein based polymer composites. Proceedings of the 1st International Conference on Natural Fibres (ICNF2013): Sustainable Materials for Advanced Applications, pp 333. Fangueiro R. (Ed.), Guimarães, Portugal. ISBN: 978-989-20-3872-8.

Collins T., Azevedo-Silva J., da Costa A., Branca F., Machado R. and Casal M. (2013). Batch production of a silk-elastin-like protein in E. coli BL21(DE3): key stress factors and parameters for optimisation. Microb. Cell Fact., Feb 27; 12:21. DOI: 10.1186/1475-2859-12-21.

Machado M., Correia C., Azevedo-Silva J, Collins T, Arias J, Rodríguez-Cabello J.C. and Casal M. (2013). High level expression and facile purification of recombinant silk-elastin-like polymers in auto induction shake flask cultures. AMB Express, Feb 5; 3(1):11. DOI: 10.1186/2191-0855-3-11.

Pereira A.M., Bernardo T.C., Costa A., Collins T., Machado R., Leonor I.B., Casal M. and Reis R.L. (2013). Development of new chimeric proteins for tissue engineering. J. Tissue Eng. Regen. Med., 7: 1, 46.

Collins T., Feller G., Gerday C. and Meuwis M.A. (2012). Family 8 enzymes with xylanolytic activity. US8309336B2.

Dutron A., Georis J., Genot B., Dauvrin T., Collins T., Hoyoux A. and Feller G. (2011). Use of family 8 enzymes with xylanolytic activity in baking. EP1549147B1(2011), US8192772(2012), CN1681392B(2010), WO2004/023879, PCT/BE03/00152; Granted and published in Europe (all countries in Europe), United States, Mexico, Japan, Eurasia, China, Brazil, Australia.

Collins T., Matzapetakis M. and Santos H. (2010). Backbone and side chain 1H, 15N and 13C assignments for a thiol-disulphide oxidoreductase from the Antarctic bacterium Pseudoalteromonas haloplanktis TAC125, Biomol. NMR Assign, Vol. 4, No. 2, 151-154.

Marx J.C., D'Amico S., Collins T. and Feller G. (2009). Cold-Adapted Enzymes: Fundamentals and Biotechnological Aspects. In Meshram, S. U. and Shinde, G. B. (eds.), Applied Biotechnology. I.K. International Publishing House Pvt. Limited, pp. 183-188. ISBN: 9380026560, 9789380026565.

Collins T., Roulling F., Florence P., Marx J.C., Feller G., Gerday C. and D’Amico S. (2008). Fundamentals of cold-adapted enzymes. In Margesin R., Schinner F., Marx J.C. and Gerday C. (Eds.), Psychrophiles: From Biodiversity to Biotechnology, Springer-Verlag, Heidelberg, Germany. Ch. 13, pp. 211-227.

Collins T., D'Amico S., Marx J., Feller G. and Gerday C. (2007). Cold-Adapted Enzymes. In Gerday, C. and Glansdorff, N. (eds.), Physiology and biochemistry of extremophiles, ASM Press, Washington, D.C., pp. 165-179.

Marx J.C., Collins T., D'Amico S., Feller G. and Gerday C. (2007). Cold-Adapted Enzymes from Marine Antarctic Microorganisms. Mar. Biotechnol. (NY), Vol. 9, 293–304.

Collins T., Hoyoux A., Van Petegem F., De Vos D., Van Beeumen J., Stals I., Nerinckx W., Claeyssens M., Georis J., Dauvrin T., Arnaut F., Gerday C. and Feller G. (2007.) A novel family 8 psychrophilic xylanase: fundamentals and application. In JAMSTEC, E.R.C. (Ed.), Proceedings of the International Symposium on Extremophiles and their Applications 2005. Japan Science and Technology Information Aggregator, Electronic (J-STAGE), Tokyo, Japan, pp. 73-79. DOI: 10.11490/isea.2005.0.73.0

Gerday C., D’Amico S., Collins T., Marx J.C., Feller G. and Sonan G. (2007). Adaptation strategies and uses of cold adapted enzymes in biotechnological processes. In JAMSTEC, E.R.C. (Ed.), Proceedings of the International Symposium on Extremophiles and their Applications 2005. Japan Science and Technology Information Aggregator, Electronic (J-STAGE), Tokyo, Japan, pp. 66-72. DOI: 10.11490/isea.2005.0.66.0

Collins T., D'Amico S., Georlette D., Marx J.C., Huston A.L. and Feller G. (2006). A nondetergent sulfobetaine prevents protein aggregation in microcalorimetric studies. Anal. Biochem. 352, 299-301.

De Vos D., Collins T., Nerinckx W., Savvides S.N., Claeyssens M., Gerday C., Feller G. and Van Beeumen J. (2006). Oligosaccharide binding in family 8 glycosidases: crystal structures of active-site mutants of the beta-1,4-xylanase pXyl from Pseudoaltermonas haloplanktis TAH3a in complex with substrate and product. Biochemistry, 45, 4797-4807.
Note: De Vos D. and Collins T. contributed equally to this work i.e. joint first authors.

D'Amico S., Collins T., Marx J.C., Feller G. and Gerday C. (2006). Psychrophilic microorganisms: challenges for life. EMBO Rep., 7, 385-389.
Note: All authors contributed equally to this work

Collins T., Hoyoux A., Dutron A., Georis J., Genot B., Dauvrin T., Arnaut F., Gerday C. and Feller G. (2006). Use of glycoside hydrolase family 8 xylanases in baking. JCS, 43, 79-84.

Collins T., De Vos D., Hoyoux A., Savvides S.N., Gerday C., Van Beeumen J. and Feller G. (2005). Study of the active site residues of a glycoside hydrolase family 8 xylanase. J. Mol. Biol., 354, 425-435.

Georis J., Dauvrin T., Hoyoux A., Collins T. and Feller G. (2005). Novel xylanases and their use. World Intellectual Property Organization, PCT, WO 2005/087916 A2.

Collins T., Gerday C. and Feller G. (2005). Xylanases, xylanase families and extremophilic xylanases. FEMS Microbiol. Rev., 29, 3-23.

D’Amico S., Georlette D., Collins T., Feller G. and Gerday C. (2005). Microcalorimetry as applied to psychrophilic enzymes. In Ladbury, J.E. (ed.), Biocalorimetry 2: Application of Calorimetry in the Biological Sciences. Wiley J. and Sons Ltd., The Atrium, Chichester, U.K., pp. 231-240. ISBN: 0470011114, 9780470011119, doi: 10.1002/0470011122.ch13

Hoyoux A., Blaise V., Collins T., D'Amico S., Gratia E., Huston A.L., Marx J.C., Sonan G., Zeng, Y., Feller G. and Gerday C. (2004). Extreme catalysts from low-temperature environments. J. Biosci. Bioeng., 98, 317-330.

Marx J.C., Blaise V., Collins T., D'Amico S., Delille D., Gratia E., Hoyoux A., Huston A.L., Sonan G., Feller G. and Gerday C. (2004). A perspective on cold enzymes: current knowledge and frequently asked questions. Cell. Mol. Biol. (Noisy-le-grand), 50, 643-655. doi: 10.1170/T554

Georlette D., Blaise V., Collins T., D'Amico S., Gratia E., Hoyoux A., Marx J.C., Sonan G., Feller G. and Gerday C. (2004). Some like it cold: biocatalysis at low temperatures. FEMS Microbiol. Rev., 28, 25-42.

Collins T., Meuwis M.A., Gerday C. and Feller G. (2003). Activity, stability and flexibility in glycosidases adapted to extreme thermal environments. J. Mol. Biol., 328, 419-428.

Van Petegem F., Collins T., Meuwis M.A., Gerday C., Feller G. and Van Beeumen J. (2003). The structure of a cold-adapted family 8 xylanase at 1.3 A resolution. Structural adaptations to cold and investigation of the active site. J. Biol. Chem., 278, 7531-7539.
Note: Van Petegem, F. and Collins T. contributed equally to this work i.e. joint first authors.

Van Petegem F., Collins T., Meuwis M.A., Gerday C., Feller G. and Van Beeumen J. (2002). Crystallization and preliminary X-ray analysis of a xylanase from the psychrophile Pseudoalteromonas haloplanktis. Acta Crystallogr. D Biol. Crystallogr., 58, 1494-1496.

Collins T., Meuwis M.A., Stals I., Claeyssens M., Feller G. and Gerday C. (2002). A novel family 8 xylanase: functional and physico-chemical characterization. J. Biol. Chem., 277, 35133-35139.

Collins T., Claverie P., D'Amico S., Georlette D., Gratia E., Hoyoux A., Meuwis M.A., Poncin J., Sonan G., Feller G. and Gerday C. (2002). Life in the cold: psychrophilic enzymes. In Pandalai, S.G. (ed.), Recent Research Developments in Proteins. Transworld Research Network, Trivandrum, Vol. 1, pp. 13-26. ISBN: 81-7895-063-4.

D'Amico S., Claverie P., Collins T., Georlette D., Gratia E., Hoyoux A., Meuwis M.A., Feller G. and Gerday C. (2002). Molecular basis of cold adaptation. Philos. Trans. R. Soc. Lond. B Biol. Sci., 357, 917-925.

Georlette D., Bentahir M., Claverie P., Collins T., D’Amico S., Delille D., Feller G., Gratia E., Hoyoux A., Lonhienne T., Meuwis M.A., Zecchinon L. and Gerday C. (2002). Cold-adapted enzymes. In Bulte J. and Decuyper M. (eds.), Physics and Chemistry Basis of Biotechnology. Vol. 7 of Series Focus on Biotechnology, Kluwer Academic Publishers B.V., pp. 177-196. doi: 10.1007/0-306-46891-3_7.

Zecchinon L., Claverie P., Collins T., D'Amico S., Delille D., Feller G., Georlette D., Gratia E., Hoyoux A., Meuwis M.A., Sonan G. and Gerday C. (2001). Did psychrophilic enzymes really win the challenge? Extremophiles, 5, 313-321.

D'Amico S., Claverie P., Collins T., Feller G., Georlette D., Gratia E., Hoyoux A., Meuwis M.A., Zecchinon L. and Gerday C. (2001). Cold-adapted enzymes: an unachieved symphony. In Storey K.B. and Storey J.M. (eds.), Cell and Molecular Responses To Stress, Vol. 2, Protein Adaptations and Signal Transduction. Elsevier, Amsterdam., Vol. 3, pp. 31-42.

Gerday C., Aittaleb M., Bentahier M., Chessa J.P., Claverie P., Collins T., D'Amico S., Dumont J., Garsoux G., Georlette D., Hoyoux A., Lonhienne T., Meuwis M.A. and Feller G. (2000). Cold-adapted enzymes: from fundamentals to biotechnology. Trends Biotechnol., 18, 103-107.