Machado Raul

Raúl Machado

raulmachado@bio.uminho.pt

People Detail

Address : Campus de Gualtar

Phone Number : 253601519

Research Area : Bio-Inspired Systems

Position : Integrated members

Ongoing Projects

Principal Investigator
• ECOBiB: Eco-Innovative Bag-In-Box Solutions. Co-promotion project from Agência Nacional de Inovação under the Compete2020 programme, reference POCI-01-0247-FEDER-033997, involving the participation of University of Minho, Pólo de Inovação em Engenharia de Polímeros (PIEP) and the industrial partner Vizelpas, Lda. (total budget 794.061,13 €; UMinho budget 167.571,76 €). Principal Investigator from UMinho. (2018-2021)
• FunBioPlas: Novel synthetic biocomposites for biomedical devices. ERA-IB (European Research Area - Industrial Biotechnology), reference ERA-IB-2-6/0004/2014 (2016-2019), funded by FCT (UMinho budget 200.000,00 €). Consortium involving University of Minho (PT), University of Valladolid (SP) and the SME, Oxford Biomaterials Ltd (UK). Principal Investigator and Coordinator. (2016-2019).
• Fun-Silk: Functionalized silk-based polymers as advanced biomaterials. CBMA project funded by the Centre of Molecular and Environmental Biology (CBMA), reference PEst-OE/BIA/UI4050/2014. (2014).

Team Member
• FUN2CYT: Harnessing the potential for biomedical applications of pleiotropic cytokines LIF and oncostatin M., funded by FCT, reference PTDC/BTM-MAT/30568/2017 (budget 235.833,12). Co-Principal Investigator. (2018-2021).
• CONCERT: Silk COated honeycomb Nanocarriers for canCER Therapy, funded by FCT, reference PTDC/NAN-MAT/32651/2017 (budget 238.120,65 €). Team member. (2018-2021).
• EcoAgriFood: Innovative Green Products and Processes to Promote Agri-Food Bioeconomy, reference NORTE-01-0145-FEDER-000009, co-funded by NORTE2020 through “Fundo Europeu de Desenvolvimento Regional” (FEDER) (total budget 1.289.747,00 €). Team Member and Task Leader. (2016-2019).
• EvoMod: Origin and Evolutionary establishment of a transcriptional module controlling flower asymmetry. Funded by FCT, reference PTDC/BIA-PLA/1402/2014 (budget 196.716,00 €). Team Member. (2016-2019).

Concluded Projects

Collaborator
• EcoPlast: Research in new biomass-based composites from renewable resources with improved properties for vehicle parts moulding”. Reference FP7 NMP 2009 SME-3 Collaborative Project number 246176, (2010-2014).
• CHIMERA: Development of a new chimeric proteins to be used in the fabrication of a novel biomaterial for bone regeneration. Reference PTDC/EBB-EBI/109093/2008, (2010-2013).

Students

PhD students - ongoing
▷ Ana Margarida Macedo Bernardes Pereira (2015-2019). Insights into the mechanism of antimicrobial activity and its exploitation for developing new therapies against clinically relevant bacteria. Doctoral Programme in Applied and Environmental Microbiology (DP_AEM), University of Minho, Braga, Portugal.
▷ Anabela Ferreira Gonçalves (2019-2023). Oncostatin M in psoriasis: a multifaceted cytokine able to modulate inflammation. FCT Scholarship with reference SFRH/BD/146807/2019, University of Minho, Braga, Portugal.

MSc students - ongoing
▷ Pedro Rafael Magalhães Veloso (2019-2020). Formulation of a drug delivery platform for the release of 5-aminosalicylic acid in the gastrointestinal tract. Master Course in Biophysics and Bionanosystems, University of Minho, Braga, Portugal.
▷ Catarina Pinheiro Macedo (2018-2019). Development of recombinant protein-based materials with ability to bind to cells and to the extracellular matrix. Master Course in Molecular Genetics, University of Minho, Braga, Portugal.
▷ Luane Gomes (2017-2018). To kill or not to kill? A question to filamentous fungi. Master Course in Molecular Genetics, University of Minho, Braga, Portugal.

MSc students - concluded
▷ Mafalda Braga Gomes (2018-2019). Recombinant protein-based hydrogels for biotech applications. Master Course in Molecular Genetics, University of Minho, Braga, Portugal, final grade of 18 over 20.
▶ João Fernando Machado Alves (2019). Development of a molecular biology platform for the production of antimicrobial peptides. Master Course in Molecular Genetics, University of Minho, Braga, Portugal, final grade of 19 over 20.
▶ Rita da Conceição Carvalho Araújo (2019). To kill or not to kill? A question to yeast. Master Course in Applied Biochemistry, University of Minho, Braga, Portugal, final grade of 18 over 20.
▶ Julie Pjipers (2017). Encapsulation of essential oils in liposomes and chitosan for dermal applications. Master in Drug Development, ERASMUS program, collaboration with University of Ghent, Belgium (Faculteit Farmaceutische Wetenschappen), final grade of 14 over 20.
▶ Diana Cristina Serra Gomes (2017). Formulation of biocomposites based on recombinant proteins and plant essential oils for the development of active wound dressings. Master Course in Plant Molecular Biology, Biotechnology and Bioentrepreneurship (M3BP), University of Minho, Braga, Portugal, final grade of 19 over 20.
▶ Andreia Maria Azevedo da Silva (2015). Production of silk-elastin-like composite materials with antimicrobial properties. Master Course in Applied Biochemistry, University of Minho, Braga, Portugal, final grade of 19 over 20.
▶ Rute Malarranha Chitas (2014). A molecular biotechnology approach for the development of Silk-Elastin-Like Polymers functionalized with Leukemia Inhibitory Factor. Master Course in Molecular Genetics, University of Minho, Braga, Portugal, final grade of 17 over 20.

BSc students - concluded
▶ Inês Andreia Costa Mesquita (2017). Péptidos Inseticidas – A sua diversidade e possíveis aplicações biotecnológicas. Graduation in Biochemistry, University of Minho, Braga, Portugal, final grade of 17 over 20.
▶ Rafaela Malta (2017). Desenvolvimento de nanofibras microbicidas pela técnica de electrospinning. Graduation in Applied Biology, University of Minho, Braga, Portugal, final grade of 19 over 20.
▶ Ana Sofia Gonçalves Abreu (2016). A Natureza como fonte de inspiração para a formulação de bio-adesivos. Graduation in Applied Biology, University of Minho, Braga, Portugal, final grade of 19 over 20.
▶ Simão Rodrigues (2014). Polímeros de base proteica recombinantes: desenvolvimento e aplicações. Graduation in Applied Biology, University of Minho, Braga, Portugal, final grade of 16 over 20.

Research Fellows - ongoing
● Diana Cristina Serra Gomes. Project ECOBiB
● Ana Margarida Macedo Bernardes Pereira. Project FunBioPlas
● António Miguel Araújo Rego. Project EcoAgriFood

Publications

Scientific Publications

24. Machado R., da Costa A., Pereira A.M., Rodriguez-Cabello J.C., Casal M. (2020). Elastin-based antimicrobial particles for delivery of bioactive compounds. Methods in Molecular Biology. Methods in Molecular Biology, 2118, 24-43. DOI: 10.1007/978-1-0716-0319-2_3

23. Tinoco A., Rodrigues R.M., Machado R., Pereira R.N., Cavaco-Paulo A., Ribeiro A. (2020). Ohmic heating as an innovative approach for the production of keratin films. International Journal of Biological Macromolecules, 150, 671-680. DOI: 10.1016/j.ijbiomac.2020.02.122

22. Machado R. & Azevedo A. (2020). Determinants and consequences of citizens’ E-participation: the case study of the app MyHomeCity. International Journal of E-planning Research, 9(1), 20-43. DOI: 10.4018/IJEPR.2020010102

21. Pinho V., Fernandes M., da Costa A., Machado R., Gomes A.C. (2019). Leukemia inhibitory factor: recent advances and implications in biotechnology. Cytokine & Growth Factor Reviews. DOI: 10.1016/j.cytogfr.2019.11.005

20. Costa T., Ribeiro A., Machado R., Ribeiro C., Lanceros-Mendez S., Cavaco-Paulo A.M., Almeida A., das Neves J., Lúcio M., Viseu T. (2019). Polymeric electrospun fibrous dressings for topical co-delivery of acyclovir and omega-3 fatty acids. Frontiers in Bioengineering and Biotechnology, 7, 390. DOI: 10.3389/fbioe.2019.00390

19. Faria M.J., Machado R., Ribeiro A., Gonçalves H., Real Oliveira M.E.C.D., Viseu T., das Neves J., Lúcio M. (2019). Rational Development of Liposomal Hydrogels: A Strategy for Topical Vaginal Antiretroviral Drug Delivery in the Context of HIV Prevention. Pharmaceutics, 11, 485. DOI: 10.3390/pharmaceutics11090485

18. Correia D.M., Ribeiro S., da Costa A., Ribeiro C., Casal M., Lanceros-Méndez S., Machado R. (2019). Development of bio-hybrid piezoresistive nanocomposites using silk-elastin protein copolymers. Composites Science and Technology, 172, 134-142.
DOI: https://doi.org/10.1016/j.compscitech.2019.01.017

17. Fernandes M.M., Correia D.M., da Costa A., Ribeiro S., Casal M., Lanceros-Méndez S., Machado R. (2018) Multifunctional magnetically responsive biocomposites based on genetically engineered silk-elastin-like protein. Composites Part B: Engineering, 153, 413-419.
DOI: https://doi.org/10.1016/j.compositesb.2018.09.019

16. da Costa A., Pereira A.M., Gomes A.C., Rodríguez-Cabello J.C., Casal M., Machado R. (2018). Production of bioactive Hepcidin by recombinant DNA tagging with an elastin-like recombinamer. New Biotechnology, 46, 45-53.
DOI: https://doi.org/10.1016/j.nbt.2018.07.001

15. Machado R., da Costa A., Silva D.M., Gomes A.C., Casal M., Sencadas V. (2018). Antibacterial and antifungal activity of poly(lactic acid)-bovine lactoferrin nanofiber membranes. Macromolecular Bioscience, 18(3), 1700324.
DOI: https://doi.org/10.1002/mabi.201700324

14. da Costa A., Pereira A.M., Gomes A.C., Rodríguez-Cabello J.C., Sencadas V., Casal M., Machado R. (2017). Single step fabrication of antimicrobial fibre mats from a bioengineered protein-based polymer. Biomedical Materials, 12(4), 045011.
DOI: https://doi.org/10.1088/1748-605X/aa7104

13. Pereira A.M., Machado R., da Costa A., Ribeiro A., Collins T., Gomes A.C., Leonor I. B., Kaplan D.L., Reis R.L., Casal M. (2017). Silk-based biomaterials functionalized with fibronectin type II promotes cell adhesion. Acta Biomaterialia, 47(1), 50-59
DOI: https://doi.org/10.1016/j.actbio.2016.10.002

12. Barroca M., Rodrigues P., Sobral R., Costa M.M.R., Chaves S.R., Machado R., Casal M., Collins T. (2016). Antibiotic free selection for the high level biosynthesis of a silk-elastin-like protein. Scientific Reports, 6, 39329.
DOI: https://doi.org/10.1038/srep39329

11. Machado R., da Costa A., Sencadas V., Pereira A.M., Collins T., Rodríguez‐Cabello J.C., Lanceros-Méndez S., Casal M. (2015). Exploring the Properties of Genetically Engineered Silk‐Elastin‐Like Protein Films. Macromolecular Bioscience, 15(12), 1698-1709.
DOI: https://doi.org/10.1002/mabi.201500132

10. Padrão J., Machado R., Casal M., Lanceros-Méndez S., Rodrigues L.R., Dourado F., Sencadas V. (2015). Antibacterial performance of bovine lactoferrin-fish gelatine electrospun membranes. International Journal of Biological Macromolecules, 81, 608-614.
DOI: https://doi.org/10.1016/j.ijbiomac.2015.08.047

9. da Costa A., Machado R., Ribeiro A., Collins T., Thiagarajan V., Neves-Petersen M.T., Rodríguez-Cabello J.C., Gomes A.C., Casal M. (2015). Development of elastin-like recombinamer films with antimicrobial activity. Biomacromolecules, 16(2), 625-635.
DOI: https://doi.org/10.1021/bm5016706

8. Collins T., Barroca M., Branca F., Padrão J., Machado R., Casal M. (2014). High level biosynthesis of a silk-elastin-like protein in E. coli. Biomacromolecules, 15(7), 2701-2708.
DOI: https://doi.org/10.1021/bm5005564

7. Machado R., da Costa A., Sencadas V., Garcia-Arévalo C., Costa C.M., Padrão J., Gomes A.C., Lanceros-Méndez S., Rodríguez-Cabello J.C., Casal M. (2013). Electrospun silk-elastin-like fibre mats for tissue engineering applications. Biomedical Materials, 8(6), 065009.
DOI: https://doi.org/10.1088/1748-6041/8/6/065009

6. Collins T., Azevedo-Silva J., da Costa A., Branca F., Machado R., Casal M. (2013). Batch production of a silk-elastin-like protein in E. coli BL21 (DE3): key parameters for optimisation. Microbial Cell Factories, 12, 21.
DOI: https://doi.org/10.1186/1475-2859-12-21

5. Machado R., Azevedo-Silva J., Correia C., Collins T., Arias F.J., Rodríguez-Cabello J.C., Casal M. (2013). High level expression and facile purification of recombinant silk-elastin-like polymers in auto induction shake flask cultures. AMB Express, 3(1), 11.
DOI: https://doi.org/10.1186/2191-0855-3-11

4. Machado R., Bessa P.C., Reis R.L., Rodríguez-Cabello J.C., Casal M. (2012). Elastin-based nanoparticles for delivery of bone morphogenetic proteins. Methods in Molecular Biology, 906, 353-363.
DOI: http://doi.org/10.1007/978-1-61779-953-2_29

3. Bessa P. C., Machado R., Nürnberger S., Dopler D., Banerjee A., Cunha A. M., Rodríguez-Cabello J.C., Redl H., van Griensven M., Reis R.L., Casal M. (2010). Thermoresponsive self-assembled elastin-based nanoparticles for delivery of BMPs. Journal of controlled release,142(3), 312-318.
DOI: https://doi.org/10.1016/J.JCONREL.2009.11.003

2. Araújo R., Silva C., Machado R., Casal M., Cunha A. M., Rodriguez-Cabello J. C., Cavaco-Paulo, A. (2009). Proteolytic enzyme engineering: a tool for wool. Biomacromolecules, 10(6), 1655-1661.
DOI: https://doi.org/10.1021/bm9002943

1. Machado R., Ribeiro A. J., Padrão J., Silva D., Nobre A., Teixeira J.A., Arias F.J., Cunha A.M., Rodríguez-Cabello J.C., Casal M. (2009). Exploiting the sequence of naturally occurring elastin: construction, production and characterization of a recombinant thermoplastic protein-based polymer. Journal of Nano Research, 6, 133-145.
DOI: https://doi.org/10.4028/www.scientific.net/JNanoR.6.133