Margarida Casal

mcasal@bio.uminho.pt

People Detail

Address : CBMA

Research Area : Bio-Inspired Systems

Position : Integrated members

Qualifications

MARGARIDA CASAL is Full Professor at the University of Minho. She obtained her degree in Biology from Faculty of Sciences of the University of Lisbon and Ph.D. degree in Biology from University of Minho. She was Director of the Department of Biology, member of the General Council, Dean of the School of Sciences and Vice Rector for Education. As Director of the Center of Molecular and Environmental Biology (2013-16) the Center was evaluated in 2013 with Excellent. She is coordinator of the doctoral program FCT in Applied and Environmental Microbiology.

Research

M. Casal academic profile can be checked in: Researcher Id: B-6386-2009 / Scopus Id: 7102313058 / ORCID: 0000-0002-0143-9758 / Ciência ID: C415-042A-1C05/ http://scholar.google.pt/citations?user=02W9yO4GjtsC&;hl=pt-PT&oi=ao; M. Casal scientific interests are focused on protein structural-functional analysis with the goal of understanding the molecular features that allow proteins to perform work in the cell. Currently the main scientific interests are focused in two model proteins: i) PLASMA MEMBRANE TRANSPORTERS. By using an assortment of biological materials, covering bacteria, yeasts or human cells, the aim is to solve the 3D structure of carboxylic acid permeases and understand how they work and selectively transport the substrates eg lactate, malate, citrate. ii) RECOMBINANT PROTEIN-BASED POLYMERS. The aim is to design, produce, characterize and develop applications for Recombinant Protein-based Polymers based on the structure of silk and elastin blocks, providing an extraordinary tool to create self-structured and “smart” (stimuli responsive) biopolymers.

Ongoing Projects

Yeast Biotechnology Doctoral Training Programme (YEASTDOC). GA” No. 764927, “Marie Sklodowska-Curie European Joint Doctorate Programme – EJD”. 2017-21. Global funding: 3004944,48€, UMinho funding: 414 072,72€. Coordenadora UMinho.
EcoAgriFood: Innovative green products and processes to promote Agri-Food BioEconomy. Referência: NORTE-01-0145-FEDER-000009. Data de inicio: 01/06/2016. Data de fecho: 31/05/2020. Orçamento total: 1.289.747,00€. Coordenadora.
TransAcids – Structural-functional studies of carboxylic acid transporters: Novel tools in production optimization using industrial microbes /Estudos estrutura-função de transportadores de ácidos carboxílicos: novas ferramentas na otimização da produção de estirpes microbianas industriais. PTDC/BIA-MIC/5184/2014. Funding: €199.620,00. Junho 2016-2020. Coordenadora.
FunBioPlas - Novel synthetic biocomposites for biomedical devices. ERA-NET IB-2 project FunBioPlas (grant number ERA-IB-15-089) with FCT reference ERA-IB-2-6/0004/2014, EuroNanoMed 2016 call Funding: €200.000,00. Junho 2016-2020. Membro da equipa.
Single Cause Single Cure Foundation - February 2016-20. 25 K dollars. Coordenadora.

Publications

Last 5 years:
2016
- Azevedo-Silva J, Queirós O, Baltazar F, Ulaszewski S, Goffeau A, Ko Y H, Pedersen P L, Preto A, Casal M. 2016. The Anticancer Agent 3-Bromopyruvate: A Simple But Powerful Molecule Taken From The Lab To The Bedside. J Bioenerg Biomembrane 48, 349–362.
- Ferro, S., Azevedo-Silva, J., Casal, M., Côrte-Real, M., Baltazar, F., Preto, A. 2016. Characterization of acetate transport in colorectal cancer cells and potential therapeutic implications Oncotarget 7 (43), 70639-70653
- Barroca, M., Rodrigues, P., Sobral, R., Costa, M.M., Chaves, S., Machado, R., Casal, M., Collins, T. 2016. Antibiotic free selection for the high level biosynthesis of a silkelastin-like protein. Scientific Reports. 6: 39329.DOI: 10.1038/srep39329.
2017
- Pereira, A.M., Machado, R., da Costa, A., Ribeiro, Collins, T., Gomes, A. C., Leonor, I., Kaplan, D. L., Reis, R.L., Casal, M. Silk-based biomaterials functionalized with fibronectin type II promotes cell adhesion. Acta Biomaterialia, 47, 50–59
- da Costa, André, Pereira, A., Gomes, A., Rodriguez-Cabello, J., Sencadas, V., Casal, M., Machado, R. 2017. Single step fabrication of antimicrobial fibre mats from a bioengineered protein-based polymer. BMM-101735.R1
- Ribas, D., Sá-Pessoa, J., Soares-Silva, I., Paiva, S., Nygard, Y., Ruohonen, L., Penttilä, M., Casal, M. 2017. Yeast as a tool to express sugar acid transporters with biotechnological interest FEMS Yeast Research, 17, 1-17
- Alves, R., Mota, S., Silva, S., Rodrigues, Célia F., Brown, Alistair J P., Henriques, M., Casal, M., Paiva, S. 2017. The carboxylic acid transporters Jen1 and Jen2 affect the architecture and fluconazole susceptibility of Candida albicans biofilm in the presence of lactate. Biofouling, 1-12.
- Talaia, G., Gournas, C., Saliba, E., Barata-Antunes, C., Casal, M., André, B., Diallinas, G., Paiva, S. 2017. The α-Arrestin Bul1p Mediates Lactate Transporter Endocytosis in Response to Alkalinization and Distinct Physiological Signals. Journal of Molecular Biology, Volume 429: 3678-3695
2018
- Machado, R., da Costa, A., Silva, D.M., Gomes, A.C., Casal, M., Sencadas, V. 2018. Antibacterial and antifungal activity of poly(lactic acid)-bovine lactoferrin nanofiber membranes. Macromolecular Bioscience. doi: 10.1002/mabi.201700324. [Epub ahead of print]
- da Costa, A., Pereira, A.M., Gomes, A.C., Rodriguez-Cabello, J.C., Casal, M., Machado, R. 2018. Production of bioactive hepcidin by recombinant DNA tagging with an elastin-like recombinamer. New Biotechnolgy, 46, 45-53.
- Fernandes, M.M.. da Costa, A. Casal, M., Lanceros-Méndez, S. Machado, R. Multifunctional magnetically responsive biocomposites based on genetically engineered silk-elastin-like protein. Journal: Composites Part B, 153, 413-419.
- Gomes, S.D., Oliveira, C.S., Azevedo-Silva, J., Casanova, M., Barreto, J., Pereira, H., Chaves, S., Rodrigues, L., Casal, M., Corte-Real, M., Baltazar, F., Preto, A. 2018. The Role of Diet Related Short-Chain Fatty Acids in Colorectal Cancer Metabolism and Survival: Prevention and Therapeutic Implications. Current Medicinal Chemistry. DOI : 10.2174/0929867325666180530102050
2019
- Ko, Y., Niedźwiecka, K. Casal, M. Pedersen, P.L., Ułaszewski, S. 2019. 3‐Bromopyruvate as a potent anticancer therapy in honor and memory of the late Professor André Goffeau. Yeast 36 (4), 211-221
- Ribas, D. Soares-Silva, I., Vieira, D., Sousa-Silva, M., Sá-Pessoa, J., Azevedo-Silva, J., Viegas, C., Arraiano, M.C., Diallinas, G., Paiva, S., Soares, P., Casal, M., 2019. The acetate uptake transporter family motif “NPAPLGL(M/S)” is essential for substrate uptake. Fungal Genetics and Biology, 122, 1-10
- Correia, D.M., Ribeiro, S., da Costa, A., Ribeiro, C., Lanceros-Mendez, S., Machado, R. 2019. Development of bio-hybrid piezoresistive nanocomposites using silk-elastin protein copolymers. Composites Science and Technology 172 134–142.
- Pereira-Vieira, J., João Azevedo-Silva, J., Preto, A., Casal, M., Queirós, O. 2019. MCT1, MCT4 and CD147 expression and 3-bromopyruvate toxicity in colorectal cancer cells are modulated according to the extracellular conditions. Biological Chemistry 400 (6), 787-799
- Niedźwiecka, K., Ribas, D., Casal, M., Ułaszewski, S. 2019. The Cryptococcus neoformans monocarboxylate transporter Jen4 is responsible for increased 3-bromopyruvate sensitivity. FEMS yeast research 19 (3), foz029
2020
- Alves, R., Barata-Antunes, C., Casal, M., Brown, AJP., Van Dijck, P., Paiva, S. 2020. Adapting to survive: how Candida overcomes host-imposed constraints during human colonization. Plos Pathogens. 16(5): e1008478. https://doi.org/10.1371/journal.ppat.1008478
- Alves, R., Sousa-Silva, M., Vieira, D, Soares, P., Chebaro, Y., Lorenz, M., Casal, M., Soares-Silva, I., Paiva, S. 2020. Carboxylate transportes in Candida pathogenesis. mBio. 11:e00156-20. https://doi.org/10.1128/mBio.00156-20.
- Alves, R., Kastora, S.L., Gomes-Gonçalves, A., Azevedo, N., Rodrigues, C.F., Silva, S., Demuyser, L., Van Dijck, P., Casal, M., Brown, AJP., Henriques, M, Paiva, S. 2020. Transcriptional responses of Candida glabrata biofilm cells to fluconazole are modulated by the carbon source. NPJ Biofilms Microbiomes. 6:4. doi: 10.1038/S41522-020-0114-5.
- Machado, R., da Costa, A., Pereira, A.M., Rodriguez-Cabello, J. Casal, M. 2020 Elastin-Based Antimicrobial Particles for Delivery of Bioactive Compounds. Nanoparticles in Biology and Medicine pp 29-43.
- Soares-Silva, I., Ribas, D.; Sousa-Silva, M., Silva, J.; Rendulić, T., Casal, M. 2020 Membrane transporters in the bioproduction of organic acids: state of the art and future perspectives for industrial applications. FEMS Microbiology Letters, 367, 2020, fnaa118