People Detail

Associate Professor w/ Habilitation at the Department of Biology, University of Minho. Course Director of the PhD Program in Molecular and Environmental Biology. PhD in Biomedical Sciences – Microbiology at ICBAS, University of Porto. Graduation in Biochemistry at University of Porto.

Her main areas of interest have been the cellular and molecular responses of yeast to different environmental stresses or changes, including metabolic adaptation with emphasis on transport systems, mechanisms underlying resistance or induction of cell death. She has been especially involved in the study of yeast apoptotic and necrotic pathways and their regulation, using acetic acid and ammonium as death stimuli. In addition, she uses yeast as a model system for the study of heterologously expressed mammalian proteins and for the identification of cellular targets of natural compounds and novel synthetic molecules. . She has been particularly interested in the development of studies addressing the exploitation of non-conventional yeast in biotechnology, using approaches at the biochemical and physiological levels but also at the genomic, transcriptomic and metabolomics levels.

Omics approaches towards the industrial exploitation of Torulaspora delbrueckii: Elucidation of the molecular basis underlying complex cellular traits. Leading Institution UM: PI - Maria João Sousa. (2018- 2021)

Advancing wine yeast genomics - exploring the evolutionary dimensions of domestication and the emergence of virulence. PTDC/BIA-MIC/30785/2017. Leading Institution UNL: PI - José Paulo Sampaio. Coordination at UM – Maria João Sousa. (2018 -2021)

A functional approach to unravel the interaction between fungicide pollution and fungi mediated ecosystem processes. PTDC/CTA-AMB/29505/2017. Leading Institution UM: PI - Bruno Castro. Team member. (2018-2021)

SOURDOMICS – Sourdough biotechnology network towards novel, healthier and sustainable food and bioprocesses. CA18101. COST Action Association. 2018-22.

Essential oils, infusions, and silicon in crop protection. PTDC/BAA-AGR/31131/2017. Leading Institution UP: PI - Manuel Ferreira. (2018-2021)

YEASTDOC H2020 Marie Sklowodska-Curie European Joint Doctoral Programme in Yeast Biotechnology. (1 set 2017-). Leading Institution UCC, Irlanda: PI - John Morissey. Team member. (2017-2020)

NEWFOOD – Food Technologies Valorization (NORTE-01-0246-FEDER-000043) Leading Institution UTAD: PI - Alberto Baptista. (2017-2019)

CLUSTER TRANSFRONTERIZO DE BIOTECNOLOGIA. Consolidacion y competitividad del sector biotecnologico transfronterizo. (Operación 0082_CLUSTERBIOTRANSFRONTERIZO_1_P) – Projeto financiado pelo Programa de Cooperación Interreg V-A España – Portugal (POCTEP) 2014-2020. Leading Institution UM: PI - José Couto Teixeira. Team member. (2017-2019)

“HEALTHKAKI”, project n.º Nº 010672, co-financed by Fundo Europeu de Desenvolvimento Regional – FEDER, through COMPETE, Programa Operacional Factores de Competitividade.
Leading Institution UM: PI - Prof. Alberto Dias. Team member. (2016-2018)
Phosphoregulation of Bax-dependent cell death. FCT-ANR/BEX-BCM/0175/2012. Portuguese PI - Prof. Manuela Côrte-Real, Universidade do Minho. Team member. (2013-2016)
Role of the yeast neutral sphingomyelinase in mitochondria function and iron homeostasis. PTDC/BBB-BQB/1850/2012. PI - Prof. Vitor Costa, ICBAS/IBMC, Universidade do Porto. Team member. (2013-2015)
Identification of molecular components involved in the mitochondrial membrane permeabilization during programmed cell death: role of the ANT, VDAC and ceramides. Acções Universitárias Integradas Luso-Francesas/PAUILF, (Acção Nº F-TC-6/10). PI - Prof. Manuela Côrte-Real, Universidade do Minho. Team member. (2010-2011)

Investigating the role of cofilin in mitochondrial permeabilisation and apoptosis. Acções Integradas Luso-Britânicas 2010, CRUP (Proc.º B-35/10). PI - Prof. Manuela Côrte-Real, Universidade do Minho. Team member. (2010-2011)

ZygoSacAR-Mecanismos de resistência a ácidos fracos em leveduras contaminantes alimentares: do modelo experimental Saccharomyces cerevisiae a Zygosaccharomyces spp. PTDC/AGR-ALI/102608/2008. PI - Prof. Isabel Sá-Correia, Universidade Técnica de Lisboa. Team member. (2010-2013)

Elucidation of ceramide-induced apoptosis: modulation of Protein Kinase C isoforms., PTDC/BIA-BCM/69448/2006. PI - Prof. Manuela Côrte-Real, Universidade do Minho. Team member. (2009-2012)

Implementation of a Nacional Facility for DNA Microarrays: Phase II. PTDC/BIA-BCM/64745/2006. PI - Prof. Manuel Santos, Universidade de Aveiro. Team member. (2009-2012)

New bio-products and technologies for valorization of Hypericum species (HypericumBiotech.) PTDC/AGR-AAM/70418/2006. PI - Prof. Manuel Ferreira, Universidade do Minho. Team member. (2008-2011)
Caracterização da diversidade fenotípica e genotípica dos isolados da colecção de leveduras do Instituto dos Vinhos do Douro e Porto (Financiamento IVDP) 2008-2011. Projeto desenvolvido no âmbito de um “Contrato de Investigação e Desenvolvimento” entre a Universidade do Minho e o IVDP. PI at UM – Maria João Sousa.

BYZYMO – INVESTIGAÇÃO E DESENVOLVIMENTO EM LEVEDURAS - Integrou a equipa promotora do spin-off académico, 2006-2010.

Evaluating the Antimicrobial Actions of Potential new Food Preservatives - Projeto com financiamento do CRUP no âmbito do Programa Luso-Britânico de Investigação Conjunta - Tratado de Windsor / Acções Integradas Luso-Britânicas, 2006. PI at UM – Maria João Sousa. Responsábel pela participação británica Prof. Peter Piper, Universidade de Sheffield.

FreezYeast - Projeto no âmbito do Concurso de Ideias de Negócio Five promovido pelo IAPMEI em colaboração com a AdI, 2005. Financiado igualmente pelo Programa NEOTEC da AdI, 2006. Integrou a equipa promotora do projeto.

Estudo dos mecanismos envolvidos na via apoptótica mitocondrial induzida pelo ácido acético em Saccharomyces cerevisiae - Projeto em curso desde 2003, financiamento interno pelo CB. Este projeto recebeu igualmente financiamento do GRICES, para deslocação de investigadores a França, 2004 e 2005, 2007 e 2008. PI - Prof. Manuela Côrte-Real, Universidade do Minho.
Paragens de fermentações alcoólicas – causas múltiplas e soluções integradas. Projeto financiado pela FCT no âmbito do Programa POCTI, 2003 a 2006. Responsável pelo projeto no Departamento de Biologia. PI - Prof. Cecília Leão, Universidade do Minho.

Caracterização dos mecanismos de transporte e utilização de açúcares em Torulaspora delbrueckii. Avaliação da sua capacidade fermentativa em massas panares. Este projeto recebeu igualmente financiamento do CRUP e do GRICES, para deslocação de investigadores a Espanha nos anos de 2002 e 2003.


Valorização da Salvia sp, Melissa sp, Mentha sp e Lavandula sp, para obtenção de produtos de valor acrescentado, com actividade biológica - Projeto financiado pela FCT no âmbito do Programa POCTI, 2001 a 2003. PI - Prof. Manuel Ferreira, Universidade do Minho.
Actividade antimicrobiana de novos derivados de imidazola - Projeto com financiamento interno pelo CB e financiamento FCT (SFRH/BD/3185/2000), 2000-2005. Responsável pelo projeto no Departamento de Biologia, UM.

Remoção de ácido acético de mostos e vinhos azedos - Projeto financiado pela FCT no âmbito do Programa PRAXIS XXI, 1999 a 2001. PI - Prof. Cecília Leão, Universidade do Minho.

Utilização de Fungicidas em Vitivinicultura: Efeitos em Leveduras e Fermentações Vinárias - Projeto financiado pelo INIA no âmbito do programa PAMAF, 1997 a 2000. PI - Prof. Cecília Leão, Universidade do Minho.

Isolamento e caracterização de leveduras: aplicações em enologia - Projeto financiado pelo INIA no âmbito do programa PAMAF, 1996 a 1999. PI - Prof. Cecília Leão, Universidade do Minho.

Spoilage yeasts in food and beverages: Characterization and ecology for improved diagnosis and culture – Projeto financiado pela CE, 1994 a1999. PI - Prof. Cecília Leão, Universidade do Minho.

Caracterização química e actividade farmacológica de plantas da família Compositae existentes em Trás-os-Montes - 1987-1988 (4 meses). PI - Prof. José Magalhães Cardoso, Universidade do Porto.

PhD Students

João Carlos Canossa Ferreira, sob o tema “Uncovering cell death induction mechanisms and anti-cancer properties of novel benzo[a]phenoxazine derivatives” Co-orientação com: Prof. Ana Preto (DB,UM) e Maria Sameiro Gonçalves (DQ, UM).

Joana Isabel Carvalho Pereira, sob o tema "Development of a new antifungal strategy based on encapsulated antimicrobial peptides: evaluation of the mechanism of action and in vivo efficiency on Candida systemic infections" Co-orientação com: Prof. Paula Sampaio (DB, UM).

Anabela Araújo Ferreira, sob o tema “The role of Bcl-2 protein family and Beclin-1 in KRAS-mediated regulation of apoptosis/autophagy using yeast and colorectal cancer as models”, Co-orientação com: Prof. Ana Preto (DB, UM).

Lorena Donzella, sob o tema “Enhancing yeast sugar transport capacity under stress conditions”, Co-orientação com: Dr John Morrissey (University College Cork, Irlanda).

Luis Pedro de Matos Melo Ferraz, sob o tema “Membrane stress caused by short chain fatty acids in Saccharomyces cerevisiae”, Co-orientação com: Prof. Paola Branduardi (Università degli Studi di Milano-Bicocca, Itália).

Master Students

Ana Rita Santos Nunes no âmbito do Mestrado em Genética Molecular, sob o tema “Molecular targets underlying benzo[a]phenoxazines antifungal activity: the role of ergosterol metabolism and endoplasmic reticulum stress”, Universidade do Minho. Co-orientação: Prof. Sameiro Gonçalves (Dep. Química, UM).
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Martins, V.M., Fernandes, T.R., Lopes, D., Domingues M.R.M., Côrte-Real, M. and Sousa, M.J. (2019) Contacts in Death: The Role of the ER–Mitochondria Axis in Acetic Acid-Induced Apoptosis in Yeast. Journal of Molecular Biology 431(2):273-288. doi: 10.1016/j.jmb.2018.11.002. Epub 2018 Nov 8.

Costa, A.R., Machado, N., Rego, A., Sousa, M.J., Côrte-Real, M., Chaves, S.R., (2019) Proteasome inhibition prevents cell death induced by the chemotherapeutic agent cisplatin downstream of DNA damage, DNA Repair (Amst) 73:28-33. doi: 10.1016/j.dnarep.2018.10.006. Epub 2018 Oct 25.

Bettencourt, A.P., Castro, M., Silva, J.P., Fernandes, F., Coutinho, O.P., Sousa, M.J., Proenca, M.F., Areias, F.M. Phenolic Imidazole Derivatives with Dual Antioxidant/Antifungal Activity: Synthesis and Structure-Activity Relationship. Med Chem. 2018 Oct 5. doi: 10.2174/1573406414666181005143431. [Epub ahead of print]

Bettencourt, A., Castro, M., Silva, J., Fernandes, F., Coutinho, O., Sousa, M.J., Proença, M.F., Areias, F. (2018) New Nitrogen Compounds Coupled to Phenolic Units with Antioxidant and Antifungal Activities: Synthesis and Structure-Activity Relationship. Molecules 23(10). pii: E2530. doi: 10.3390/molecules23102530.

Cazzanelli, G., Pereira, F., Alves, S., Francisco, R., Azevedo, L., Dias Carvalho, P., Almeida, A., Côrte-Real, M., Oliveira, M.J., Lucas, C., Sousa, M.J.*, Preto, A.* (2018) The Yeast Saccharomyces cerevisiae as a Model for Understanding RAS Proteins and their Role in Human Tumorigenesis. Cells 7(2). pii: E14. doi: 10.3390/cells7020014. *Co-last authors

Carmona-Gutierrez, D. et al. (2018) Guidelines and recommendations on yeast cell death nomenclature. Microbial Cell, Vol. 5, No. 1, pp. 4 - 31; DOI: 10.15698/mic2018.01.607

Mendes, I., Sanchez, I., Franco-Duarte, R., Camarasa, C., Schuller, D., Dequin, S., Sousa, M.J. (2017) Integrating transcriptomics and metabolomics for the analysis of the aroma profiles of Saccharomyces cerevisiae strains from diverse origins. BMC Genomics 18(1):455. doi: 10.1186/s12864-017-3816-1.

Trindade, D., Pereira, C., Chaves, S.R., Manon, S., Côrte-Real, M., Sousa M.J. (2016) VDAC regulates AAC-mediated apoptosis and cytochrome c release in yeast. Microbial Cell 3, pp.369-379.

Leitão, M.I.P.S., Raju, B.R., Naik, S., Coutinho, P.J.G., Sousa, M.J., Gonçalves, M. S. T. (2016) Synthesis and photophysical studies of new benzo[a]phenoxazinium chlorides as potential antifungal agents Tetrahedron Letters, Volume 57, Issue 35, Pages 3936-394.

Santos, J., Leitão-Correia, F., Sousa, M.J. *, Leão, C. (2016), Nitrogen and carbon source balance determines longevity, independently of fermentative or respiratory metabolism in the yeast Saccharomyces cerevisiae. Oncotarget. 2016 Apr 8. 7(17):23033-42. doi: 10.18632/oncotarget.8656. [Epub ahead of print] *Co-last author and author for correspondence.

Klionsky, D.J. et al (2016) Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy. 2016;12(1):1-222.

Simonyan, L., Renault, T. T., da Costa Novais, M. J., Sousa, M. J., Côrte-Real, M., Camougrand, N., Gonzalez, C., Manon, S. (2016), Regulation of Bax/mitochondria interaction by AKT. FEBS Letters, 590: 13–21. doi: 10.1002/1873-3468.12030.

Santos, J., Leitão-Correia1, F., Sousa, M.J.* and Leão, C. (2016) Dietary restriction and nutrient balance in aging. Oxidative Medicine and Cellular Longevity 4010357. doi: 10.1155/2016/4010357 *Co-last author and author for correspondence.

Alves, S., Castro, L., Fernandes, M., Francisco, R., Castro, P., Priault, M., Chaves, S., Moyer, M., Oliveira, C., Seruca, R., Côrte-Real, M., Sousa, M.J.* and Preto, A. (2015) “Hotspot mutations in the KRAS oncogene function as positive regulators of autophagy.” Oncotarget 6(31):30787-802. doi: 10.18632/oncotarget.5021. *Co-last author.

Santos, J., Sousa, M.J.* and Leão, C. (2015) Ammonium is a key determinant on the dietary restriction of yeast chronological aging in culture medium. Oncotarget 6(9):6511-23. *Co-last author and author for correspondence.

Rego, A., Duarte, A. M., Azevedo, F., Sousa, M.J., Côrte-Real M. and Chaves, S.R. (2014). Cell wall dynamics modulate acetic acid-induced apoptotic cell death of Saccharomyces cerevisiae. Microbial Cell 1(9): 303-314.

Ferreira, P., Cardoso, T., Ferreira, F., Fernandes-Ferreira, M., Piper, P. and Sousa, M.J. (2014) Mentha piperita essential oil induces apoptosis in yeast associated with both cytosolic and mitochondrial ROS-mediated damage. FEMS Yeast Res. 14(7):1006-14. doi: 10.1111/1567-1364.12189. Epub 2014 Aug 26.

Mira, N.P., Münsterkötter, M., Dias-Valada, F., Santos, J., Palma, M., Roque, F.C., Guerreiro, J.F., Rodrigues, F., Sousa, M.J., Leão, C., Güldener U. and Sá-Correia, I. (2014) The genome sequence of the highly acetic acid-tolerant Zygosaccharomyces bailii derived interspecies hybrid strain ISA1307, isolated from a sparkling wine plant. DNA Res 21 (3): 299-313. doi:10.1093/dnares/dst058.

Rego, A., Trindade, D., Chaves, S.R., Manon, S., Costa, V., Sousa, M.J. and Côrte-Real, M. (2014) The yeast model system as a tool towards the understanding of apoptosis regulation by sphingolipids. FEMS Yeast Research 14(1), 160–178. DOI: 10.1111/1567-1364.12096.

Sousa, M., Duarte, A.M., Fernandes, T. R., Chaves, S.R., Pacheco, A., Leão, C., Côrte-Real M. and Sousa, M.J. (2013) Genome-wide identification of genes involved in the positive and negative regulation of acetic acid-induced programmed cell death in Saccharomyces cerevisiae. BMC Genomics, 14:838.

Pacheco, A., Azevedo, F., Rego, A., Santos, J., Chaves, S.R., Côrte-Real, M. and Sousa, M.J. (2013) C2-phytoceramide perturbs lipid rafts and cell integrity in Saccharomyces cerevisiae in a sterol-dependent manner. PLOS ONE 8(9):e74240. doi: 10.1371/journal.pone.0074240.

Santos, J., Leão, C. and Sousa, M.J. (2013) Ammonium-Dependent Shortening of CLS in Yeast Cells Starved for Essential Amino Acids Is Determined by the Specific Amino Acid Deprived, through Different Signaling Pathways. Oxidative Medicine and Cellular Longevity, vol. 2013, Article ID 161986, 10 pages, 2013. doi:10.1155/2013/161986.

Pereira, H., Azevedo, F., Rego, A., Sousa, M.J., Chaves, S.R. and Côrte-Real, M. (2013) The protective role of yeast cathepsin D in acetic acid-induced apoptosis depends on ANT (Aac2p) but not on the voltage-dependent channel (Por1p). FEBS Letters 587(2):200-5. DOI: 10.1016/j.febslet.2012.11.025.

Rodrigues, F., Sousa, M.J., Ludovico, P., Santos, H., Côrte-Real, M., Leão, C. (2012) The fate of acetic acid during glucose co-metabolism by the spoilage yeast Zygosaccharomyces bailii. PLoS One 7(12):e52402. doi: 10.1371/journal.pone.0052402.

Rego, A., Costa, A.M., Chaves, S.R., Pereira, H.P., Sousa, M.J., Moradas-Ferreira P., Costa, V. and Côrte-Real, M. (2012) Modulation of mitochondrial outer membrane permeabilization and apoptosis by ceramide metabolism. PLoS ONE 7(11): e48571. doi:10.1371/journal.pone.0048571.

Santos, J., Leão, C. and Sousa, M.J. (2012) Growth culture conditions and nutrient signaling modulating yeast chronological longevity. Oxid Med Cell Longev. 2012:680304. doi: 10.1155/2012/680304.

Santos, J., Sousa, M.J.* and Leão, C. (2012) Ammonium is toxic for aging yeast cells, inducing death and shortening of the chronological lifespan. PLOS ONE 7(5):e37090. doi: 10.1371/journal.pone.0037090. *Co-last author and author for correspondence.

Sousa, M.J., Azevedo, F., Pedras, A., Marques, C., Coutinho, O.P, Preto, A., Gerós, H., Chaves, S.R. and Côrte-Real, M. (2011) Vacuole-mitochondrial cross-talk during apoptosis in yeast: a model for understanding lysosomal-mitochondrial-mediated apoptosis in mammals. Biochem. Soc. Trans. 39, 1533-1537.

Vilela-Moura, A., Schuller, D., Mendes-Faia, A., Silva, R.D., Chaves, S.R., Sousa, M.J. and Côrte-Real, M. (2011) The impact of acetate metabolism on yeast fermentative performance and wine quality: reduction of volatile acidity of grape musts and wines. Appl Microbiol Biotechnol 89, 271–280.

Pereira, C., Chaves, S., Alves, S., Salin, B., Camougrand, N., Manon, S., Sousa, M.J. and Côrte-Real, M. (2010) Mitochondrial degradation in acetic acid-induced yeast apoptosis: the role of Pep4 and the ADP/ATP carrier. Mol Microbiol 76(6), 1398-410.

Pacheco, A., Pereira, C., Almeida, M.J. and Sousa, M.J. (2009) Small heat shock protein Hsp12 contributes to yeast tolerance to freezing stress. Microbiology 155, 2021-8.

Pacheco, A., Almeida, M.J. and Sousa, M.J. (2009) Improved gene disruption method for Torulaspora delbrueckii. FEMS Yeast Res 9(1), 158-60.

Pereira, C., Silva, R.D., Saraiva, L., Johansson, B., Sousa, M.J. and Côrte-Real, M. (2008) Mitochondria dependent apoptosis in yeast. Biochim Biophys Acta 1783(7), 1286-302.

Frade, V.H.J., Sousa, M.J., Moura, J.C.V.P. and Gonçalves, M.S.T. (2008) Synthesis of naphtho[2,3-a]phenoxazinium chlorides. Structure-activity relationships of these heterocycles and benzo[a]phenoxazinium chlorides as new antimicrobials. Bioorg Med Chem 16 (6), 3274-3282.

Santos, J., Sousa, M.J., Cardoso, H., Inácio, J., Silva, S., Spencer-Martins, I. and Leão, C. (2008) Ethanol tolerance of sugar transport and the rectification of stuck wine fermentations. Microbiology 154, 422-30.

Frade, V.H.J., Sousa, M.J., Moura, J.C.V.P. and Gonçalves, M.S.T. (2007) Synthesis, characterisation and antimicrobial activity of new benzo[a]phenoxazine based fluorophores. Tetrahedron Lett 48, 8347–8352.

Pereira, C., Camougrand, N., Manon, S., Sousa, M.J. and Côrte-Real, M. (2007) ADP/ATP carrier is required for mitochondrial outer membrane permeabilization and cytochrome c release in yeast apoptosis. Mol Microbiol 66(3), 571-82. Commented at Molecular Microbiology, Micro Commentary by Lawen A. Another piece of the puzzle of apoptotic cytochrome c release, 66(3):553-6.

Conde, C., Silva, P., Fontes, N., Dias, A.C.P., Tavares, R.M., Sousa, M.J., Agasse, A., Delrot, S. and Gerós, G. (2007) Biochemical changes throughout grape berry development and fruit and wine quality. Food 1(1), 1-22.

Alves-Araújo, C., Pacheco, A., Almeida, M.J., Spencer-Martins, I., Leão, C. and Sousa, M.J. (2007) Sugar utilization patterns and respiro-fermentative metabolism in the baker’s yeast Torulaspora delbrueckii. Microbiology 153, 898-904.

Guaragnella, N., Pereira, C., Sousa, M.J., Antonacci, L., Passarella, S., Côrte-Real, M., Marra, E. and Giannattasio, S. (2006) YCA1 participates in the acetic acid induced programmed cell death also in a manner unrelated to its caspase-like activity. FEBS Lett 580(30), 6880-6884.

Alves-Araújo, C., Hernandez-Lopez, M.J., Prieto, J.A., Randez-Gil, F. and Sousa, M.J. (2005) Isolation and characterization of the LGT1 gene encoding a low-affinity glucose transporter from Torulaspora delbrueckii. Yeast 22, 165-75.

Alves-Araújo, C., Almeida, M.J., Sousa, M.J. and Leão, C. (2004) Freeze tolerance of the yeast Torulaspora delbrueckii: cellular and biochemical basis. FEMS Microbiol Lett 240(1), 7-14.

43. Alves-Araújo, C., Hernandez-Lopez, M.J., Sousa, M.J., Prieto, J.A. and Randez-Gil, F. (2004) Cloning and characterization of the MAL11 gene encoding a high-affinity maltose transporter from Torulaspora delbrueckii. FEMS Yeast Res 4, 467-476.

Rodrigue, F., Zeeman, A.M., Cardoso, H., Sousa, M.J., Steensma, H.Y., Côrte-Real, M. and Leão, C. (2004) Isolation of an acetyl-CoA synthetase gene (ZbACS2) from Zygosaccharomyces bailii. Yeast 21(4), 325-31.

Araújo, C., Sousa, M.J., Ferreira, M. and Leão, C. (2003) Activity of essential oils from Mediterranean Lamiaceae species against food spoilage yeasts. J Food Prot 66(4), 625-32.

Rodrigues, F., Ludovico, P., Sousa, M.J., Steensma, Y., Côrte-Real, M. and Leão, C. (2003) The spoilage yeast Zygosaccharomyces bailii forms mitotic spores: A screening method for haploidization. Appl Environ Microbiol 69(1), 649-53.

Ludovico, P., Sousa, M.J., Silva, M.T., Leão, C. and Côrte-Real, M. (2001) Saccharomyces cerevisiae commits to a programmed cell death process in response to acetic acid. Microbiology 147, 2409-15.

Rodrigues, F., Zeeman, A.M., Alves, C., Sousa, M.J., Steensma, H.Y., Côrte-Real, M. and Leão, C. (2001) Construction of a genomic library of the food spoilage yeast Zygosaccharomyces bailii and isolation of the ß-isopropylmalate dehydrogenase gene (ZbLEU2). FEMS Yeast Research 1(1), 67-71.

Ribeiro, I.C., Veríssimo, I. Moniz, L., Cardoso, H., Sousa, M.J., Soares, A.M.V.M. and Leão, C. (2000) Yeasts as a model for assessing the toxicity of the fungicides penconazol, cymoxanil and dichlofluanid. Chemosphere 41, 1637-1642.

Prudêncio, C., Sansonetty, F., Sousa, M.J., Côrte-Real, M. and Leão, C. (2000) Rapid detection of efflux pumps and their relation with drug resistance in yeast cells. Cytometry 39, 26-35.

Sousa, M.J., Rodrigues, F., Côrte-Real, M. and Leão, C. (1998) Mechanisms underlying the transport and intracellular metabolism of acetic acid in the presence of glucose by the yeast Zygosaccharomyces bailii. Microbiology 144, 665-670.

Sousa, M.J., Miranda, L., Côrte-Real, M. and Leão, C. (1996) Transport of acetic acid in Zygosaccharomyces bailii: effects of ethanol and their implications on the resistance of the yeast to acid environments. Appl Environ Microbiol 62, 3152-3157.

Sousa, M.J., Mota, M. and Leão, C. (1995) Effects of ethanol and acetic acid on the transport of malic acid and glucose in the yeast Schizosaccharomyces pombe: implications in wine deacidification. FEMS Microbiol Lett 126, 197-202.

Sousa, M.J., Teixeira, J.A. and Mota, M. (1993) Must deacidification with an induced flocculant yeast strain of Schizosaccharomyces pombe. Appl Microbiol Biotechnol 39(2), 189-193.

Sousa, M.J., Mota, M. and Leão, C. (1992) Transport of malic acid in the yeast Schizosaccharomyces pombe: evidence for a proton dicarboxilate symport. Yeast 8(12), 1025-1031.

Sousa, MJ., Teixeira, J.A. and Mota, M. (1992) Differences in the flocculation mechanism of Kluyveromyces marxianus and Saccharomyces cerevisiae. Biotechnol Lett 14 (3), 213-218.

Sousa, M.J., Teixeira, J.A. and Mota, M. (1991) Malo-alcoholic fermentation: the influence of operating conditions on the kinetics of deacidification. Journal of Wine Research 2 (2), 115-124.